Journal of Plant Molecular Breeding

Genetic diversity of Satureja bachtiarica Bunge species collected from north-west Iran

Document Type : Original research paper

Author

Plant Science and Medicinal Plants, University of Mohagheghe Ardabili.

Abstract

Sustained availability of genetic resources is essential for successful plant breeding. Satureja bachtiarica Bunge is an endemic species to Iran, widely dispersed throughout the country. The rocky mountainous terrain between Asalem and Khalkhal, situated in the Ardabil province, is one of them. The current research focused on the genetic classification of 11 different ecotypes of S. bachtiarica collected from northwest Iran using RAPD molecular markers. A significant genetic diversity was observed among the ecotypes, with 64 bands displaying substantial polymorphism. These polymorphic bands served as the foundation for genetic analyses conducted using NTYSYS-pc (2.02e) software. The genetic distance between the ecotypes was determined using the Dice similarity coefficient. Subsequently, a dendrogram was constructed based on the similarity matrix data, employing the unweighted pair-group method with arithmetic averages (UPGMA). Genetic clustering analysis of the molecular marker data from 11 studied ecotypes revealed the lowest genetic similarity among the Khoy and Ardabil ecotypes. In contrast, the Khalkhal and Meshgin Shahr ecotypes exhibited the highest similarity. After examining the dendrogram, it becomes clear that most clusters consist of ecotypes sharing entirely similar or relatively similar climatic conditions. This suggests that the molecular diversity outcomes align with the geographical diversity of the ecotypes.

Keywords

Main Subjects

Arzani, A., and Ashraf, M. (2016). Smart engineering of genetic resources for enhanced salinity tolerance in crop plants. Crit Rev Plant Sci 35(3): 146-189.
Azad, H.N., and Nematadeh, G. (2013). Introducing a new method of genomic DNA extraction in dicotyledonous plants. Sch J Agric Sci 2: 242-248.
Baba Nitsa, M., Odiyi, A.C., Akinyele, B.O., Aiyelari, O.P., and Fayeun, l.S. (2023). Genetic diversity assessment of thirty nine Coffea canephora accessions using EST-SSR markers. J Plant Mol Breed 11(1): 17-27. doi: 10.22058/jpmb.2023.2002371.1273.
El Hafid, R., Blade, S., and Hoyano, Y. (2002). Seeding date and nitrogen fertilization effects on the performance of borage (Borago officinalis L.). Ind Crops Prod 16(3): 193-199.
Fathi, R., Mohebodini, M., and Chamani, E. (2021). Evaluation of genetic diversity of summer savory (Satureja hortensis L.) accessions based on morphological and phytochemical characteristics. Int J Med Aromat Plants Res 36(6): 1005-1021.
Fathimoghaddam, E., Shakerian, A., Sharafati Chaleshtori, R., and Rahimi, E. (2020). Chemical composition and antioxidant properties and antimicrobial effects of Satureja bachtiarica Bunge and Echinophora platyloba DC. Essential oils against listeria monocytogenes. J Medicinal Plants 9(Special): 47-58.
Hadian, J., Tabatabaei, S., Naghavi, M., Jamzad, Z., and Ramak-Masoumi, T. (2008). Genetic diversity of Iranian accessions of Satureja hortensis L. based on horticultural traits and RAPD markers. Sci Hortic 115(2): 196-202.
Heydari, A., Hadian, J., Esmaeili, H., Kanani, M.R., Mirjalili, M.H., and Sarkhosh, A. (2019). Introduction of Thymus daenensis into cultivation: Analysis of agro-morphological, phytochemical and genetic diversity of cultivated clones. Ind Crops Prod 131: 14-24.
Kameli, M., Hesamzadeh Hejazi, S., and Ebadi, M. (2013). Assessment of genetic diversity on populations of three Satureja species in Iran using ISSR markers. Ann Biol Res 4(3): 64-72.
Li, L., Li, J., Zou, L., Bai, S.-y., Niu, L.-m., and Ma, Y.-k. (2007). RAPD analysis of genetic diversity of nine strains of Auricularia auricular cultivated in Heilongjiang Province. J For Res 18: 136-138.
Monika, S., Jakub, S., Kornelia, P., Czeslaw, H., and Roman, Z. (2006). Comparison of three Polygonatum species from Poland based on DNA markers. Ann Bot Fenn 43: 378-388.
Namayandeh, A., Mokhtari, N., Kamelmanesh, M.M., and Bedaf, M.T. (2017). Genetic diversity in Satureja species determined by sequence-related amplified polymorphism markers. J Genet 96: 5-11.
Peiri, T., and Fazeli, A. (2022). Identification of ecotypes and different species of savory (Satureja spp.) using DNA barcoding genes. J Veg Sci 6(1): 82-96.
Saidi, M., Movahedi, K., Mehrabi, A.A., and Kahrizi, D. (2013). Molecular genetic diversity of Satureja bachtiarica. Mol Biol Rep 40: 6501-6508.
Salehi, M., Arzani, A., Talebi, M., and Rokhzadi, A. (2018). Genetic diversity of wheat wild relatives using SSR markers. Genetika 50(1): 131-141.
Soleimani, M., Arzani, A., Arzani, V., and Roberts, T.H. (2022). Phenolic compounds and antimicrobial properties of mint and thyme. J Herb Med 36: 100604.
Zhang, G.-m., Li, Y.-R., He, W.-Z., He, H., Liu, X.-H., Song, H.-Z., Liu, H.-B., Zhu, R.-C., and Fang, W.-K. (2010). Analysis of the genetic diversity in Saccharum spontaneum L. accessions from Guangxi Province of China with RAPD-PCR. Sugar Tech 12: 31-35.
Journal of Plant Molecular Breeding
Volume 11, Issue 2
June 2023
Pages 31-38
  • Receive Date: 11 January 2024
  • Revise Date: 02 February 2024
  • Accept Date: 31 January 2024
  • First Publish Date: 31 January 2024