The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.)

Document Type : Original research paper

Authors

1 Horticultural Department, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

Dog rose (Rosa canina L.) is one of the most important ornamental and medicinal plants which are used as a rootstock for ornamental roses such as Rosa hybrid and Rosa floribunda. In vitro propagation of rose has a very important role in rapid multiplication of species with desirable traits and in production of healthy and disease-free plants. Micropropagation of Rosa canina L. was revised, using its nodal segments under different combinations of BAP (0, 0.5, 1, 1.5 and 2 mgl1), GA3 (0 and 0.5 mgl1) and NAA (0 and 0.5 mgl1) on Murashige and Skoog (MS) and Van der Salm medium (VS) in proliferation stage and using different combinations of NAA and IBA (0, 0.3, 0.6 and 0.9 mgl1) and ½ VS medium in rooting stage. The highest shoot proliferation was obtained on VS medium containing 2 mgl1 BAP. Furthermore, the highest root induction obtained in ½ VS containing 0.6– 0.9 mgl1 of NAA or IBA. The present study presents an in vitro protocol for R. canina.

Keywords

- References
[1]        Arteca, R. 1990. Hormonal stimulation of ethylene biosynthesis. In Polyamines and Ethylene: Biochemistry, Phisiology and Interactions, American Society of Plant Phisiologists, Rockville, MD, 216–223.
[2]        Carelli, BP. and Echeverrigaray, S. 2002. An improves system for the in vitro propagation of rose cultivars. Sci Hortic, 92: 69-74.
[3]        Dixon, RA. and Gonzales, RA. 1993. Plant Cell Culture. A practical approach. 2nd Edn. Plant Biology division. The Samuel Roberts Noble Foundation. P.O.Box 2180. Ardmore, Oklahoma 73402, USA (At Oxford University Press. Oxford, New York Tokyo).
[4]        Horn, W. 1992. Micropropagation of rose (Rosa L.). Biotechnol Agric Springer, (20): 320-342.
[5]        Hyndman, S., Hasegawa, P.M. and Bressan, R.A. 1982. The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots. Plant Cell Tissue Organ Cult, (1): 229–238.
[6]        Kim Chkou, J.U., Jee, S.O. and Chung, J.D. 2003. In vitro Micropropagation of Rosa hybrid L.J. Plant Biotechnol, 5: 115-119.
[7]        Kirichenko, E.B., Kuz-Mina, T.A. and Kataeva, N.V. 1991. Factors in optimizing the multiplication of ornamental and essential oil roses in vitro. Byullenten Glavnogo Bot kogo Sada, 159: 61-67.
[8]        Khosh-Khui, M. and Sink, K.C. 1982a. Micropropagation of new and old world Rosa species. Am J Hortic Sci, 57: 315-9.
[9]        Khosh-Khui, M, and Sink, K.C. 1982b. Rooting enhancement of Rosa hybrid for tissue culture propagation. Sci Hortic, 17: 371-6.
[10]      Khosh-Khui, M, and Sink, K.C. 1982c. Callus induction and culture of Rosa. Sci Hortic, 17: 361-70.
[11]      Kumar Pati, P., Prasad Rath, S., Sharma, M., Sood, A. and Singh Ahuja, P. 2006. In vitro propagation of rose– a review, Biotechnol Adv, 24: 94–114.
[12]      Lunca, A. 2005. A guide to medicinal plants in North Africa. Center for Mediterranean cooperation, Malaga (Spain), p. 256.
[13]      Motallebi-Azar, A., Shirdel, M., Masiha, S., Mortazavi, N., Maloobi, M. and Sharafi, Y. 2011. Effects of inorganic nitrogen source and NH4:NO3 ratio on proliferation of dog rose (Rosa canina L.). J Med Plants Res, 5(18): 4605-4609.
[14]      Mudge, M.W. 1989. Effect of ethylene on rooting. In adventitious root formation in cuttings, eds., TD Davis; BE Haissing and N Sankhla. Dioscorides Press. Portland, DR, pp. 150-161.
[15]      Pati, PK., Sharma, M., Sood, A., and Ahuja, PS, 2005. Micropropagation of Rosa damascena and R. bourboniana in liquid cultures. In: Hvoslef Eide AK, Preil W, editors. Liquid systems for in vitro mass propagation of plants. Neth Kluwer Academic Publishers.
[16]      Roberts, A.V. and Schum, A. 2003. Cell Tissue & Organ Culture. Encyclopedia of rose science. Oxford: Elsevior Academic Press, 57-110.
[17]      Rout, G.R. and Jain, S.M. 2004. Micropropagation of ornamental plants– cut flowers. Propagation ornam Plants, 4: 3-28.
[18]      Sharafi, Y. 2010a. Biological characteristics of pollens in some genotypes of Rosa canina L. as main factors affecting fruit set. Afr J Med Plants Res, 2 (20): 2173-2175.
[19]      Sharafi, Y. 2010b. Suitable in vitro medium for studying pollen viability in some of the Iranina hawthorn genotypes. J Med Plants Res, 4(19): 1967-1970.
[20]      Vijaya, N., Satyanarayana, G., Prakash, J., and Pierik, RLM. (1991). Effect of culture media and Growth Regulators on in vitro propagation of rose. Curr Plant Sci Biotechnol Agric, 12: 209-214.
[21]      Wissemann, V. 2003. Classification. Encyclipedia of rose science. Oxford: Elsevior Academic Press, 111-117.
[22]      Xing, W. 2010. Micropropagation of Rosa Rougosa through axillary shoot proliferation, Acta Biologica Cracoviensia. Ser Bot, 69–75.
 
 
 
 
 
Volume 3, Issue 1
June 2015
Pages 61-71
  • Receive Date: 26 August 2014
  • Revise Date: 26 August 2015
  • Accept Date: 26 August 2015
  • First Publish Date: 26 August 2015