Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)Journal of Plant Molecular Breeding2322-33324120160601Expression analysis of K+ transporter genes associated with salinity tolerance in grape192015010.22058/jpmb.2016.20150ENNayer MohammadkhaniShahid Bakeri High Education Center of Miandoab, Urmia University, Urmia, IranReza HeidariDepartment of Biology, Faculty of Science, Urmia University, Urmia, IranNasser AbbaspourDepartment of Biology, Faculty of Science, Urmia University, Urmia, IranFatemeh RahmaniDepartment of Biology, Faculty of Science, Urmia University, Urmia, IranJournal Article20151222Molecular information of K+ accumulation in grapes is strongly required. Under salinity condition potassium transporters are inhibited by Na+. The aim of this study was to investigate the effects of salinity on the expression of K+ transporter genes in grape. Based on the previous screening study on 18 grape genotypes, ‘H6’ and ‘Gharashani’ (tolerant) and ‘Shirazi’ and ‘GhezelUzum’ (sensitive) were selected. Plants were treated with 50 mM NaCl as a critical concentration that was not lethal for grapevine plants. Interestingly, the expression of VvKUP1, VvKUP2 and VvK1.1 genes highly increased in leaves of sensitive genotypes compared to tolerant ones. Also the expression of VvKUP1 and VvKUP2 genes were similar in the leaves of sensitive genotypes. There was a significant positive correlation (Phttps://www.jpmb-gabit.ir/article_20150_ba3b9476444bd3cc12c951d6570634b6.pdfGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)Journal of Plant Molecular Breeding2322-33324120160601Comparison of ISSR and AFLP markers in assessing genetic diversity among Nettle (Urtica dioica L.) populations.10161965910.22058/jpmb.2016.19659ENMostafa HaghpanahDepartment of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, Iran0000-0002-8234-7221Seyed Kamal KazemitabarDepartment of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, Iran1111-1111-1111-1111Seyed Hamidreza HashemiGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, Iran0000-0002-0870-1691Seyed Mohammad AlaviGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, IranJournal Article20151005Urtica dioica is an important medicinal plant which is widely distributed in Mazandaran province (North of Iran). In this study for the first time Amplified Fragment Length Polymorphism (AFLP) and Inter-simple Sequence Repeat (ISSR) markers were used for detection of genetic polymorphism in Mazandaran nettle. Ten AFLP primer combinations and seventeen ISSR markers were utilized. AFLP produced 830 scorable bands out of which 90.21% were polymorphic. ISSR primers amplified 234 bands, 181 being polymorphic (77.3%). Average heterozygosity for AFLP and ISSR markers were 0.25, 0.23 respectively. Marker Index obtains 22.25 for AFLP and 15.57 for ISSR. The number of cluster computed was same for both molecular makers but location of samples in branch were different. The total compare of these two marker systems shown AFLP marker was a useful tool for detection of U. dioica’sgenetic diversity. This plant is very variable and is genetically distinct in east, west and north of Mazandaran.https://www.jpmb-gabit.ir/article_19659_7e84fe0f1d821807efa0e37801b69eef.pdfGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)Journal of Plant Molecular Breeding2322-33324120160601First Report of a set of Genetic Identities in Prunus Rootstocks by SSR Markers17251966010.22058/jpmb.2016.19660ENMehrshad ZeinalabediniSystem Biology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Seed and Plant Improvement Institutes Campus, Mahdasht Road, Karaj, Iran1111-1111-1111-1111Parastoo MajidianDepartment of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, IranJalil DezhampourResearch Center of Agriculture and Natural Resources of East Azarbaijan, Azar Shahr Road, Tabriz, IranMotahareh KhakzadDepartment of Biotechnology, University of Zanjan, University Road, Zanjan, IranMaryam FarsiSystem Biology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Seed and Plant Improvement Institutes Campus, Mahdasht Road, Karaj, IranJournal Article20151111Prunus rootstocks play an important role in modern horticulture and commercial orchards owing to their responsibility for a wide range of characters from compatibility with cultivars to adaptation to biotic and abiotic stresses. In this study, Thirty Prunus rootstock samples were tested by 25 microsatellite markers in order to identify the genetic identity and relationships among them.17 SSR markers were useful in the discrimination of the samples on the basis of their unique molecular identities. Samples with similar codes such as (HS-401/HS-402/HS-403), (HS811/HS507/HS737/GF677), (HS126/HS-202), (HS-802/HS602) and (HS522/HS003/HS302) were shown mislabeled trees. Based on partial repeated bisection (RB ) data, the samples were grouped into six clusters which the largest cluster contained nine genotypes (all APPL, APU2 and APPU3). The second largest cluster consisted of eight genotypes (all AM, all APL, APU1, APU3 and APH10). APH rootstocks were placed into clusters two, three and six as well as cluster one which included only APPU rootstocks. The highest amount of the average internal similarities (Isim ) (0.973) belonged to cluster six, whereas the minimum amount of Isim (0.924) belonged to cluster three. The minimum level of the average external similarities (Esim ) was related to groups one (0.664) and six (0.638) indicating the highest genetic distance from other groups. The genetic identities and relatedness generated in this study provide a standard for further breeding attempts and will be used as a reference the cultivation of these promising newly released genotypes.https://www.jpmb-gabit.ir/article_19660_33a2abfb0ed83485a136bd3c24b02ace.pdfGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)Journal of Plant Molecular Breeding2322-33324120160601Genetic variation and association analysis of some important traits related to grain in rice (Oryza sativa L.) germplasm26341978110.22058/jpmb.2016.19781ENAli AalamiDepartment of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranMohammad AlizadehDepartment of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranReza Shirzadian-KhorramabadDepartment of Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran1111-1111-1111-1111Ali Akbar EbadiRice Research Institute of Iran, Rasht, IranHeydar AziziDepartment of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, IranJournal Article20150628The identification of genomic loci involved in control of quantitative traits receives growing attention in plant molecular breeding. The present study was carried out to evaluate the genetic variability among 48 rice genotypes and determine the genomic regions associated with ten grain related important traits. A total number of 63 alleles were detected by 18 selected SSR markers from different chromosomes with an average of 3.5 alleles per marker. A model-based Bayesian approach subdivided 48 evaluated rice genotypes into three major subgroups with the consideration of the highest value of ΔK. The mean r2 value for all loci pairs on the same chromosome was 0.053. A total of 38 significant marker-trait associations were identified (P< 0.05) that explaining more than 32% of the total variation. RM315, RM3428, RM289, RM16, RM574 and RM156 markers had highest R2 and most association with assayed traits, respectively. The findings of this study revealed association of grain properties in rice with some SSR markers that could serve as target genomic regions for further research such as MAS, fine mapping and candidate gene discovery in rice breeding programs.https://www.jpmb-gabit.ir/article_19781_d9e28af8a4ec2eb447892f22f47a13f7.pdfGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)Journal of Plant Molecular Breeding2322-33324120160601Assessment of genetic diversity and relationship of coastal salt tolerant rice accessions of Kerala (South India) using microsatellite markers35421983710.22058/jpmb.2016.19837ENJithin ThomasDepartment of Botany, Sacred Heart College, Thevara, Kochi, Kerala, IndiaV.J. DominicDepartment of Botany, Sacred Heart College, Thevara, Kochi, Kerala, IndiaJournal Article20160313Rice (Oryza sativa L.) is one of the most significant cereal crops, about 3 billion people, nearly half the world's population; depend on rice for survival and it offers up to 80% of daily energy intake in most of the Asian countries. Knowledge of the distribution, extent and pattern of genetic variation is useful for estimation of any possible loss of genetic diversity and its role in breeding programs. This work assessed the genetic diversity among 25 coastal rice populations of five regions of Kerala (South India) using 18 microsatellite markers. A mean PIC value of 0.37 and an average of 3.5 alleles per loci were observed. Mean Heterozygosity value of 0.29 and gene diversity value of 0.41 was attained. AMOVA demonstrated that genetic differentiation was significant at P< 0.001 and FST index value of 0.035 was obtained. Of the total diversity, 57.76% was attributed within individuals, 38.71% was attributed among individuals within populations and 3.53% among populations. Information regarding the amount of genetic variations in these salt tolerant coastal accessions and genetic relationship between genotypes are essential for designing effective breeding programs. Especially, to meet the differentiated goals of plant breeding such as breeding for increasing yield, wider adaptation, desirable quality, pest and disease resistance.https://www.jpmb-gabit.ir/article_19837_9afcf1a375b6957e18214765d904d3b0.pdfGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)Journal of Plant Molecular Breeding2322-33324120160601Improving Pistacia vera micropropagation: with emphasis on the efficiency of minerals, vitamins and plant growth regulators43542015610.22058/jpmb.2016.20156ENGhasemali GaroosiDepartment of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), Qazvin, Iran.Moharram-ali DelijamDepartment of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), Qazvin, Iran.Esmaeil Nezami-AlanaghDepartment of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), Qazvin, Iran.Ramin HosseiniDepartment of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), Qazvin, Iran.Journal Article20160416A complete micropropagation protocol was developed for Pistacia vera cv. Ghazvini, an important rootstock in Pistachio orchards in Iran. In present study, the efficiency of a new medium called GNH (Garoosi, Nezami and Haddad) was investigated against some standard media. Different vitamins, calcium (Ca) sources and plant growth regulators (PGRs) were tested on in vitro shoot multiplication and root induction. Results indicated that mean number of shoots (4.25 ± 0.25), and productivity (69.87 ± 9.19 mm) increased significantly when GNH medium formed the basal medium, compared to the standard media including Murashige and Skoog medium (MS), Juglans Medium (DKW), and McCown Woody Plant Medium (WPM). The most suitable concentration Ca and vitamin sources for shoot multiplication were 3.0 mM Ca gluconate and DKW-vitamins. The most suitable PGRs were a combination of 0.5 or 1.0 mg l-1 BAP and 0.1 mg l-1 IBA. The highest rooting parameters were obtained when 3.0 mM Ca gluconate or Fe-EDDHA (with 0.2 mM Fe) were incorporated into the GNH medium containing 2.0 mg l-1 α-naphthalenacetic acid (NAA). Finally, nearly 70% of the plantlets survived acclimatization in the greenhouse. The results suggested the GNH medium (supplemented with Ca gluconate and DKW-vitamins), as a considerable and specific medium for the rapid micropropagation of Pistacia vera cv. Ghazvini.https://www.jpmb-gabit.ir/article_20156_17635f8f01247220247d9ef3b20363c4.pdfGenetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU)Journal of Plant Molecular Breeding2322-33324120160601Improving Phosphorus Efficiency in Crops with Focus on Purple Acid Phosphatase: Potentials and Perspective55691966110.22058/jpmb.2016.19661ENMehdi Younessi-HamzekhanluDepartment of Agronomy and Plant Breeding, College of Abouraihan University of Tehran, IranAli Izadi-DarbandiDepartment of Agronomy and Plant Breeding, College of Abouraihan University of Tehran, IranMohammad Ali MalboobiDepartment of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, IranMohsen EbrahimiDepartment of Agronomy and Plant Breeding, College of Abouraihan University of Tehran, IranJournal Article20160118Low-phosphorus (P) stress as a key factor limiting plant growth and production is common in most agricultural soils. Most of the soil-applied phosphate will be rapidly immobilized and most of annually applied phosphate fertilizers are fixed in the soil in organic forms by adsorption, sedimentation and transformation. However, excess P application may lead to contamination of water sources by enriching of water bodies with nutrients that cause eutrophication. Thus understanding the mechanisms that are used by plants to cope with low-P stress will be supportive to develop more competent breeding and genetic engineering schemes for generating improved phosphorus efficient crops. To cope with P deficiency and maintenance of phosphate homeostasis, plants have developed different adaptive mechanisms, including alterations in root morphology, recycling of inorganic phosphate (Pi) and induction of acid phosphatases (APases). To establish these strategies, numerous genes are involved in alternative metabolism pathways that are regulated by complex Pi signaling networks. In this review, we intend to summarize current advances in research on the mechanisms of P efficient crops and its breeding strategies, with a particular emphasis on APase and root architecture roles in response to low-P stress.https://www.jpmb-gabit.ir/article_19661_4fca22add2835f16ed615c815ea3b15c.pdf